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The lens SP0 histone gene mRNA levels, as determined by RNase protection assay and ribonuclease protection assay, were significantly lower in antisense-treated explants. The SP1 cDNA was undetectable at days 1, 4, and 8 by RNAse protection assay. Both homologous and heterologous histone SP0 complementary DNAs (cDNAs) were measured after antisense treatment. To assess the role of SP1 in this process, lens explants were cultured in either control or SP1-specific antisense oligonucleotide-containing media for 8 days. The purpose of this study was to determine whether lens SP1 may play a role in regulating lens SP0 histone gene transcription. Previous studies in our laboratory identified a SP1-binding site within the SP0 histone gene promoter region. It is also dubbed in Malayalam, Tamil and Kannada language.A preliminary investigation of the role of the transcription factor SP1 in regulating histone H1 gene expression in the lens. It is said to be one of the best films of 2014.

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